Fig. 2.

Stage-specificity of the multiplex assay. Female-specific (a) and male-specific (b) transcripts are detected in high concentrations of ring stage parasites of the F12 line and the 3D7/AP2-G-GFP-DDglmS line). Mean Ct values ± 2SD for varying numbers of independent experiments: n = 2 and n = 9 for CCp4 in ring stage parasites and female gametocytes; n = 2 and n = 17 for PfMGET in ring stage parasites and male gametocytes, respectively. NA not available/not measured (samples at these concentrations were not available), ND not detected. Lines represent fitted linear regression curves