Table 1.
Primer and probe sequences for qRT-PCR assays, with references for earlier reported methods and primers
| Gene ID Name |
Fwd primer seq Rev primer seq |
Probe seq | Fluoro-phore |
|---|---|---|---|
| PF3D7_1031000 Pfs25 [36] |
GAAATCCCGTTTCATACGCTTG AGTTTTAACAGGATTGCTTGTATCTAA |
– | – |
| PF3D7_1031000 Pfs25 [34] |
6FAM-ccgtttcatacgcttgtaa-MGB | FAM | |
| PF3D7_0903800 CCp4 (MPX) |
CACATGAATATGAGAATAAAATTG* TAGGCGAACATGTGGAAAG |
AGCAACAACGGTATGTGCCTTAAAACG | Texas Red |
| PF3D7_0903800 CCp4 (qRT-PCR) |
CACATGAATATGAGAATAAAATTG* TAGGCGAACATGTGGAAAG |
– | – |
| PF3D7_1469900 PfMGET (MPX) |
CGGTCCAAATATAAAATCCTG* TGTGTAACGTATGATTCATTTTC |
CAGCTCCAGCATTAAAAACAC | FAM |
| PF3D7_1469900 PfMGET (qRT-PCR, [28]) |
CGGTCCAAATATAAAATCCTG* GTGTTTTTAATGCTGGAGCTG |
– | – |
MPX multiplex assay, qRT-PCR quantitative real time reverse transcription-PCR
*Intron-spanning