Figure 5.

BPF supplementation to SC diet causes stronger reduction of total liver lipid content, size and numbers of LDs compared to SC diet alone for 10 weeks. (A) Total lipids were extracted from 400 mg of liver tissue by Folch method and gravimetrically determined. (B) Quantification of the total area occupied by LDs in ORO-stained sections revealed a significant increase in CAF/CAF vs. other groups (# p ≤ 0.02), while BPF reduced the extent of intrahepatic fat accumulation, when compared to normocaloric diet (* p ≤ 0.02 vs. CAF/SC). Each bar represents the median of four animals ± SD. (C) Binary transformation of ORO staining using ImageJ. 100x, objective magnification; scale bar = 20 μm.