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Purification of peak 4 and peak 5 in Fraction B separated from the thallus of Pyropia yezoensis by the HPLC system. Peak 4 and Peak 5 were isolated by the HPLC system equipped with a diode array detector at 450 nm. Two Develosil C30-UG-5 columns were connected and used for carotenoid isolation. The mobile phase was methanol. The flow rate was 1.0 mL/min.
