Figure 4.

Degradation of Sargassum mcclurei fucoidan (S.m) by fucoidanase enzymes. (A) C-PAGE; and (B) size exclusion chromatography (SEC) of the products produced by: (1) FcnA2; (2) Fda2; (3) FdlA; and (4) FdlB on S. mcclurei fucoidan and molecular weight standards. The lowest band (**) of the standard (St), resulting from FFA2 treatment of fucoidan from F. evanescens, corresponds to a tetra-saccharide of (1→4)- and (1→3)-linked α-l-fucosyls with each fucosyl residue sulphated at C2; total mass has been calculated to be approximately 972 Da [27]. Reaction time was 48 h. (*) indicates an enzymatic fucoidan degradation product of either lower mass or higher charge than the lowest St band (**) compound.