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. 2018 Nov 1;16(11):422. doi: 10.3390/md16110422

Figure 5.

Figure 5

Purification and activity of enzyme FcnAΔ229. (A) SDS-PAGE indicating the expected molecular weight of 80 kDa and purity; (B) Western blot of purified FcnAΔ229. (St) is the protein plus molecular weight marker; and (C) enzyme activity by C-PAGE of (a) FcnA2 and (b) FcnAΔ229 on fucoidans from S. mcclurei, F. vesiculosus and F. evanescens. FcnA2 and FcnAΔ229 have similar profiles on F. vesiculosus and F. evanescens fucoidans. The reaction time was 24 h. The lowest band (**) of the standard (St), resulting from FFA2 treatment of fucoidan from F. evanescens, corresponds to a tetra-saccharide of (1→4)- and (1→3)-linked α-l-fucosyls with each fucosyl residue sulphated at C2; total mass has been calculated to be approximate 972 Da [27]. (*) An oligosaccharide of lower molecular weight or higher charge than the lowest band in the standard (**).