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. 2018 Nov 1;16(11):422. doi: 10.3390/md16110422

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Purification and activity of Fda1 and Fda2 deletion mutants. (A) SDS-PAGE; and (B) Western blot of purified: (1) Fda1Δ145; (2) Fda1Δ395; (3) Fda2-C-His; (4) Fda2Δ146; and (5) Fda2Δ390. (St) is the protein plus molecular weight marker. The expected sizes of the proteins were 90, 50, 125, 110, 70 kDa respectively. (C) Enzymatic S. mcclurei fucoidan (S.m) degradation by C-PAGE: (1) Fda1Δ145; (2) Fda1Δ395; (3) Fda2-His; (4) Fda2Δ146; (5) Fda2Δ390; (6) Fda2; and the standard (St) resulting from FFA2 treatment of fucoidan from F. evanescens. The lowest band (**) of the standard (St), resulting from FFA2 treatment of fucoidan from F. evanescens, corresponds to a tetra-saccharide of (1→4)- and (1→3)-linked α-l-fucosyls with each fucosyl residue sulphated at C2; total mass has been calculated to be 972 Da [27]. (*) indicates an enzymatic fucoidan degradation product of either lower mass or higher charge than the lowest St band (**) compound. The reaction time was 48 h.