Table 1.
Fucoidan-degrading enzymes, features, molecular weight and expression strains used.
| Enzyme Name/GenBank No. | Organism | Features a | Length (aa) b | Expected MW (kDa) | E. coli Expression Strains |
|---|---|---|---|---|---|
| FcnA CAI47003.1 | Mariniflexile fucanivorans SW5 | nd | 1007 | nd | Nd |
| FcnA2 | Mariniflexile fucanivorans SW5 | His6 (N-term) | 799 | 88 | BL21 (DE3) pGro7 c |
| FcnAΔ229 | Mariniflexile fucanivorans SW5 | His10 (N-term) | 720 | 80 | BL21 (DE3) pGro7 c |
| Fda1 AAO00508.1 | Alteromonas sp. SN-1009 | His10 (N-term) | 804 | 87 | BL21 (DE3) pGro7 c |
| Fda1Δ145 | Alteromonas sp. SN-1009 | His10 (N-term) and His10 (C-term) | 669 | 73 | BL21 (DE3) pGro7 c |
| Fda1Δ395 | Alteromonas sp. SN-1009 | His10 (N-term) and His10 (C-term) | 419 | 46 | BL21 (DE3) pGro7 c |
| Fda2 AAO00509.1 | Alteromonas sp. SN-1009 | His10 (N-term) | 868 | 94 | BL21 (DE3) pGro7 c |
| Fda2-His | Alteromonas sp. SN-1009 | His10 (N-term) and His10 (C-term) | 878 | 95 | BL21 (DE3) pGro7 c |
| Fda2Δ146 | Alteromonas sp. SN-1009 | His10 (N-term) and His10 (C-term) | 732 | 80 | BL21 (DE3) pGro7 c |
| Fda2Δ390 | Alteromonas sp. SN-1009 | His10 (N-term) and His10 (C-term) | 488 | 53 | BL21 (DE3) pGro7 c |
| FdlA AAO00510.1 | Flavobacterium sp. SA-0082 | His10 (N-term) | 684 | 74 | C41 (DE3) |
| FdlB AAO00511.1 | Flavobacterium sp. SA-0082 | His10 (N-term) | 692 | 76 | C41 (DE3) |
nd, not determined in this study. a Wild type signal peptide had been removed for codon-optimised synthesised construct; b Includes his-tags; c groES-groEL chaperone expressed from the pGro7 plasmid.