Fig. 1.
Foxp2 is predominantly expressed in D1R-MSNs in dorsolateral striatum and affects synaptic activity. a D1R-GFP, D2R-GFP and Foxp2 are expressed in a subset of striatal cells in juvenile (PND11-14) mice, arrows point to cells with overlapping D1R and Foxp2 expression (top row) or D2R expression without Foxp2 expression (bottom row). Scale bar 50 µm. b 83.7 ± 16% of D1R-GFP positive cells express Foxp2, compared to 16.9 ± 4% of D2R-GFP cells. N/n number of mice/number of slices. N/n = 3/12. c Striatal D1R-MSN mEPSC amplitude is decreased following reduced Foxp2 expression. Example of mEPSC activity in striatal D1R-MSNs from juvenile (PND14) Foxp2+/+ and Foxp2S321X/+ mice. Scale bar 200 ms/20 pA. Cumulative distribution of mEPSC amplitude (Foxp2+/+ = 19.4 ± 0.69 pA, Foxp2S321X/+ = 14.3 ± 0.36 pA, P < 0.0001) and frequency (Foxp2+/+ = 1.12 ± 0.1 Hz, Foxp2S321X/+ = 1.19 ± 0.11 Hz, NS) in striatal D1R-MSNs. Foxp2+/+ N/n = 3/31, Foxp2S321X/+ N/n = 3/32. d Example traces of mEPSC activity in striatal D2R-MSNs from juvenile (PND14) Foxp2+/+ and Foxp2S321X/+ mice Scale bar 200 ms/20 pA. Cumulative distribution of mEPSC amplitude (Foxp2+/+ = 15.64 ± 0.53 pA, Foxp2S321X/+ = 16.14 ± 0.62 pA, NS) and frequency (Foxp2+/+ = 0.92 ± 0.12 Hz, Foxp2S321X/+ = 0.89 ± 0.1 Hz, NS) in striatal D2R-MSNs. Foxp2+/+ N/n = 3/22, Foxp2S321X/+ N/n = 3/19. e Example traces of mIPSC activity in striatal D1R-MSNs from juvenile (PND14) Foxp2+/+ and Foxp2S321X/+ mice. Scale bar 200 ms/10 pA. Cumulative distribution of mIPSC amplitude (Foxp2+/+ = 8.6 ± 0.27 pA, Foxp2S321X/+ = 11.9 ± 0.83 pA, P < 0.001) and frequency (Foxp2+/+ = 0.18 ± 0.036 Hz, Foxp2S321X/+ = 0.71 ± 0.14 Hz, P < 0.01) in striatal D1R-MSNs. Foxp2+/+ N/n = 3/20, Foxp2S321X/+ N/n = 3/17. ***P < 0.01. N number of mice, n number of cells. All data analyzed by two-sided Students’ T test