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. 2018 Oct 24;10(11):581. doi: 10.3390/v10110581

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of exogenous application of ABA on the SMV-G7H-GFP and PP2C3a levels in L29 soybean cultivar. (A) Protein blot for SMV-G7H-GFP in response to exogenous application of ABA (100 µM) or Mock (0.1% MeOH). Upper panel is GFP level, and lower panel is ponceau-S as loading control. (B) RTqPCR of PP2C3a gene in response to SMV-G7H-GFP infection, ABA, or a mix of both. Actin11 was used as internal control. Data are means ± standard deviation from 3 biological replicates. Statistical analysis was carried out using one-sided student t test to determine the significance of the regulation compared to mock-treated plants (where * and ** indicates p < 0.05, and p < 0.01, respectively).