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. 2018 Oct 25;10(11):399. doi: 10.3390/cancers10110399

Figure 1.

Figure 1

Downregulation of tumour necrosis factor-related apoptosis-inducing ligand-receptor 1 (TRAIL-R1) decreases levels of mature miR-370-3p but not of pri-miR-370. (A) Quantitative PCR analyses detecting changes in the levels of mature miR-370-3p in Panc1 cells transfected for 40 h with a control-siRNA (ctrl.-si) or siRNA against TRAIL-R1 (TRAIL-R1-si). (B) Panc1 cells transfected with ctrl.-si were stimulated with anti-TRAIL (10 µg/mL), TRAIL (10 ng/mL) or left untreated. Levels of mature miR-370-3p were quantified by qPCR. (C,D) QPCR analyses of pri-miR-370 levels in Panc1 cells transiently transfected with ctrl.-si or TRAIL-R1-si (C) or in ctrl.-si transfected cells with and without treatment with TRAIL (10 ng/mL) or anti-TRAIL (1 µg/mL) (D). Shown are the mean ± SD of five biological replicates (n = 5), with each one analysed in technical duplicates. The asterisks (*) indicate significance (p < 0.05); n.s.: not significant.