Figure 2.

Ectopic expression of miRNA-370-3p in PDAC cells decreases the abundance of TGFβ-RII. Panc1 cells were transfected with 50 nM of an artificial miR-370-3p (miRNA-370-3p mimic) for the indicated periods of time. The levels of TGFβ-RII were analysed by Western blotting in whole cell lysates. Detection of β-actin served as a loading control. The graph underneath the blot shows results from densitometric quantification of band intensities from three independent experiments (mean ± SD, n = 3). The asterisks (*) indicate significance (p < 0.05) relative to respective untreated control.