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. 2018 Oct 25;10(11):399. doi: 10.3390/cancers10110399

Figure 3.

Figure 3

Knockdown of TRAIL-R1 increases the abundance of TGFβ-RII in Panc1 cells. Panc1 cells were transfected with siRNA against TRAIL-R1 or with control siRNA for 72 h without (A) or with (B) exposure to a neutralizing antibody against TRAIL (anti-TRAIL, 10 µg/mL) or (C) recombinant TRAIL (10 ng/mL). The expression of TRAIL-R1 and TGFβ-RII was analysed by Western blotting in whole cell lysates. As control for equal gel loading, levels of β-actin were determined in parallel. The blots shown are representative of three independent experiments yielding very similar results. (D) Densitometry-based quantification of the Western blots shown in (A). Data were compiled from three independent experiments and represent the mean ± SD (n = 3). (E) Densitometry-based quantification of the Western blots shown in (B). (F) Densitometry-based quantification of the Western blots shown in (C). The asterisks (*) in (D–F) indicate significance relative to the ctrl.-siRNA; n.s.: not significant.