Table 2.
The qPCR Assay is Specific to Swimmer’s Itch-Causing Cercariae.
| Species | qPCR Result |
|---|---|
| Trichobilharzia stagnicolae* | + |
| Trichobilharzia szidati* | + |
| Cotylurus sp. | − |
| Diplostomum baeri | − |
| Diplostomum huronense | − |
| Diplostomum indistinctum | − |
| Diplostomum sp. 1 | − |
| Diplostomum sp. 2 | − |
| Diplostomum sp. 3 | − |
| Diplostomum sp. 8 | − |
| Drepanocephalus auritus | − |
| Echinostoma caproni | − |
| Echinostoma trivolvis | − |
| Neodiplostomum americanum | − |
| Notocotylidae sp. | − |
| Haematoloechus sp. | − |
| Icthyocotylurus sp. 3 | − |
| Ornithodiplostomum sp. 8 | − |
| Plagiorchis sp. | − |
| Pseudopsilostoma varium | − |
| Strigeidae gen. | − |
| Telorchis sp. | − |
| Apharyngostrigea pipientis | − |
| Bolbophorus sp. | − |
| Tylodelphys scheuringi | − |
The qPCR assay was tested against a library of purified trematode DNA from across North America. Asterisks indicate swimmer’s itch-causing species. (+) indicates the target product amplified, while (−) indicates that no target was amplified.
*Statistically significant (P < 0.05)