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. 2018 Nov 2;10(11):1621. doi: 10.3390/nu10111621

Figure 1.

Figure 1

The effect of docosahexenoic acid (DHA) on cell viability, DNA fragmentation, protein levels of caspase-3, Bcl-2, and Bax, and the Bax/Bcl-2 ratio in pancreatic cancer cells (PANC-1) cells. Cells were treated with the indicated concentrations of DHA for 24 h and 48 h for cell viability determinations or for 24 h for measurements of DNA fragmentation, the levels of caspase-3, Bcl-2 and Bax, and the Bax/Bcl-2 ratio. (A) Cell viability measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. * p < 0.05 vs. the corresponding “None”. “None” corresponds to the untreated cell extract; “50”, “100”, and “150” correspond to the extracts of cells treated with 50, 100, and 150 µM DHA, respectively. (B) DNA fragmentation determined from the amount of oligonucleosome-bound DNA detected in the cell extracts. The description of the columns is the same as in (A). (C) Levels of capase-3, Bcl-2, and Bax in whole cell extracts determined by Western blot analysis. The description of the columns is the same as in (A). (D) The ratio of Bax/Bcl-2 determined from the Bax and Bcl-2 protein band densities. * p < 0.05 vs. “None”. The description of the columns is the same as in (A). The Bax/Bcl-2 ratio for “None” was set at 1.