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Neuronal identity. A-B) Photomicrographs of presumptive mitral/tufted cell (M/T) and interneuron (Int) in primary OB cultures. C-D) Immunolabeling of cultured OB neurons with glutamic acid decarboxylase (GAD), the GABA-synthesizing enzyme, and vesicular glutamate transporter 1 (VGLUT1) revealed that mitral/tufted cells (M/T) were VGLUT1 positive (+), while interneurons (Int) were GAD +). E-F) Photomicrographs of biocytinfilled mitral cell and external tufted (ET) cell in OB slice.
