Skip to main content
. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Neuroscience. 2018 Sep 11;391:25–49. doi: 10.1016/j.neuroscience.2018.09.002

6.

6.

A subset of KARs in the OB is located at synapses. A1, A2) To determine whether KARs in the OB are located at synapses, excitatory postsynaptic currents (EPSCs) in neurons in OB slices were evoked by stimulation of the olfactory nerve layer (ONL). AP5 (to block NMDARs) and GYKI (to block AMPARs) were then coapplied to isolate EPSCs mediated by postsynaptic KARs. Compared to the control currents, application of 100 μM AP5 + 100 μM GYKI significantly reduced the mean amplitudes of EPSCs in mitral cells (A1)(N=4; P ≤ 0.05) and JG cells (A2)(N=8; P≤ 0.05), leaving visible the KAR-mediated portions of the responses. B) Histogram of data from a subset of cells showing a significant difference in the amplitudes of EPSCs recorded in the presence of 100 μM AP5 alone (to isolate AMPA/KA receptors by blocking NMDARs) or the presence of 100 μM AP5 + 100 μM GYKI (to isolate KARs by blocking NMDARs + AMPARs) expressed as a percentage of the control EPSC amplitude (N=5; P* ≤ 0.05). Values are the mean ± SEM.