Figure 1. DIAP cells are capable of undergoing reductive cell divisions.

(a & b) Cell cycle analyses of U2932 & VAL cells respectively after treatment with 50nM alisertib for 4 days followed by recovery in the absence of drug up to 30-days shows that DLBCL cells establish a diploid 2n-near aneuploid population. (c) Polyploidy was induced by treating cells with 50nM alisertib for 4 days, then 8n-cells were sorted based on H2B-GFP expression and utilized forward scatter plot which correspond to cell size by FACS sorting followed by recovery of 8n-cells. Cell cycle analysis showed that with time 8n polyploid cells can shift back to 2n-near aneuploid cells. (e, f, g) Time-lapse single cell imaging of 8n cells (same as c), showed that polyploid cells can divide into aneuploid cells by multipolar mitosis, meiosis-like or budding-like cell division respectively compared to DMSO treated control cells by binary cell division (d).