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. 2018 Nov 13;9(89):35941–35961. doi: 10.18632/oncotarget.26310

Figure 3. Subcellular location of miR-200 precursors in tumoral pancreatic cell lines.

Figure 3

Total RNA was extracted from nuclear (N) and cytoplamic (C) fractions prepared from tumoral pancreatic cells. Low molecular weight (LW) RNAs were obtained by separation of total RNA on 8% acrylamide gel. Primary miR-200s (pri-miR-200b,a,429 and pri-miR-200c,141) were amplified from total RNA. Pre-mature miR-200s (pre-miR-429 and pre-miR-141) were amplified from LW RNAs. Note that primers for primary forms do not amplify pre-mature miR-200s. Data are representative of 2 separate experiments. RT-, negative reverse transcription.