Figure 5. Effect of RanQ69L-GTP on the interaction between XPO₅ and pre-miR-200s in tumoral pancreatic cell lines.

BxPC3 and Mia-Paca2 were pre-treated with 2 μM RanQ69L-GTP for 1 h at 37° C, before the preparation of cytoplasmic (cyto, black columns) and nuclear (nucl, grey columns) fractions. XPO₅ was immunoprecipitated from each cell compartment and co-immunoprecipitated pre-mature miRNAs were extracted then measured by RT-qPCR. In parallel, total pre-mature miRNAs level was determined prior to immunoprecipitation in both cytoplasmic and nuclear fractions. As control, immunoprecipitation with irrelevant IgG was performed. Values are the mean ± SD of triplicates. Data are representative of 2 separate experiments and are expressed as the ratio between immunoprecipitated and total pre-mature miRNA determined for each cell fraction. pre-miR-429, pre-mature miR-429; RanGTP, RanQ69L-GTP. ^**P < 0.01; ^***P < 0.001.