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. 2018 Nov 13;9(89):35941–35961. doi: 10.18632/oncotarget.26310

Figure 6. Expression of Dicer1 and MCPiP1 in tumoral pancreatic cell lines.

Figure 6

(A) MCPiP1 and Dicer1 gene expression was determined by RT-qPCR on RNA extracted from tumoral pancreatic cells. Data were normalized with the expression of the housekeeping gene 28S ribosomic RNA. (B) Dicer1 and MCPiP1 protein level was analysed by immunoblotting on cell lysates prepared from tumoral pancreatic cells. The integrated optical density of each band was quantified using ImageJ software. Values obtained for each sample were normalized for the corresponding β-actin protein level. (C) protein expression ratio between MCPiP1 and Dicer1 determined from immnublottings for all the cell lines examined.