Table 3. Expression level of miR-200 precursors in tumoral pancreatic cell lines.
| miR-200s/snU6 × 102 | ||||||||
|---|---|---|---|---|---|---|---|---|
| Mia-Paca2 | PANC1 | BxPC3 | Soj-6 | |||||
| C | N | C | N | C | N | C | N | |
| pri-miR-200b,a,429 | ND | 1.8 ± 0.60 | ND | 3.1 ± 0.71 | ND | 210 ± 57 | ND | 390 ± 49 |
| pri-miR-200c,141 | ND | 2.5 ± 0.39 | ND | 3.3 ± 0.84 | ND | 410 ± 13 | ND | 660 ± 81 |
| pre-miR-429 | ND | 0.15 ± 0.07 | ND | 0.28 ± 0.11 | 18 ± 6.3 | 41 ± 9.2 | 0.83 ± 0.22 | 64 ± 19 |
| pre-miR-141 | ND | 0.33 ± 0.09 | ND | 0.32 ± 0.09 | 23 ± 3.8 | 27 ± 7.5 | 0.63 ± 0.24 | 110 ± 21 |
The expression of primary (pri-miR) and pre-mature (pre-miR) miR-200s was determined by RT-qPCR as described in Material and Methods section, and normalized with snU6 gene expression. Data are the mean ± SD of 2 separate experiments. C, cytoplasmic compartment; N, nuclear compartment; ND, no detectable.