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. 2018 Nov 13;9(89):35891–35906. doi: 10.18632/oncotarget.26288

Figure 1. Comparison of different poxviruses in human tumor cell lines and progeny virion production.

Figure 1

(A) ED50 (MOI in pfu/cell) on a panel of tumor cell lines upon poxviruses infection at multiple MOI. Five human cancer cell lines were infected with the different viruses at several MOI from 1 to 10–5. Viability was evaluated 5 days later by trypan blue exclusion. Each experiment was done in triplicate. ED50 was determined by using the MOI which allow 50% of cell death. ED50 values greater than 10 indicate that tumor cells were insensitive to the virus at the tested MOIs. (B) Replication of RCNV, ORFV, SQFV and CTV on human glioblastoma cell line. U-87 MG cells were infected at MOI 0.01 in 6 well plates. At each time point, cells were harvested, sonicated and titrated on adequate cells. Results are expressed as viral fold increased (corresponding to output/input ratio).