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. 2018 Nov 13;9(89):35891–35906. doi: 10.18632/oncotarget.26288

Figure 3. FCU1 functionality after expression by recombinant RCNV.

Figure 3

(A) Conversion of 5-FC to 5-FU and release of 5-FU in the cell culture supernatant. LoVo cells were infected with the indicated vector at a MOI of 10–3 and then incubated with 0.3 mM 5-FC from day 2 to day 7 post infection. The detection and the relative concentration of 5-FC and 5-FU in the culture supernatant was measured by HPLC. The results are expressed as the percentage of 5-FU released relative to the total amount of 5-FC+5-FU. Each data point represents the mean of triplicate determinations ± SD. (B) In vitro sensitivities of infected human tumor cells to 5-FC. LoVo human tumor cells were infected with the RCNVwt and RCNtk-/gfp::fcu1 at a MOI of 10–4. After 48 h, cells were grown in the presence of increasing concentrations of 5-FC. Cell survival was determined 3 days later as described in Materials and Methods section. Cell viability results are expressed as the percentage of viable cells relative to untreated/non-infected cells. Each data point represents the mean of triplicate determinations ± SD.