Fig. 4.

The S117A-FGF-2 protection from Dox does not require HO-1 or CK2 activity. (a) The effect of S117A-FGF2, versus FGF2, on relative HO-1 levels in cardiomyocytes treated with Dox, as estimated by western blotting. Images from the corresponding blot probed for HO-1, or stained with Ponceau S, are also included (a′). (b) The effect of Tin-PP (HO-1 inhibitor) on S117A-FGF2 protection from Dox-induced LDH release, as indicated. Inhibition of HO-1 does not prevent S117A-FGF2 protection. (c) The effect of two CK2 inhibitors, TBB or Elagic acid, on the protective effect of S117A-FGF2, or FGF2, against Dox-induced LDH release. In the absence of inhibitors, both S117A-FGF2 and FGF2, significantly reduce Dox-induced LDH release. In the presence of either inhibitor, FGF2 is no longer protective, while S117A-FGF2 retains significant protective ability. In all panels, brackets denote groups significantly different from each other