Figure 2.

NLRP6 inhibits cell proliferation of gastric cancer cells.

Notes: (A) Overexpression of NLRP6 in BGC-823 and HGC-27 cells was confirmed by Western blot. Blots are representative of three separate experiments. (B) Cell proliferation was detected at 0, 24, 48, and 72 hours in BGC-823 and HGC-27 stable cells by CCK-8 assay (n=3). (C) Annexin V/PI assay showed that NLRP6 overexpression increased cell early and late apoptosis rates (n=3). (D) NLRP6 inhibits gastric cancer cell proliferation in vivo. BGC-823 cells stably expressed NLRP6 or control vector were subcutaneously inoculated into nude mice (six per group). Tumor volume was measured for 27 days. NLRP6-overexpressed BGC-823 cells grew much slower in nude mice than the control cells (left panels). At 27 days, mice were sacrificed and the tumors were isolated. IHC staining of Ki-67 and TUNEL assay were performed on the xenograft tumors (n=3, right panels). WT, wild-type cells; vector, cells stably expressed control vector; NLR6, cells stably expressed NLRP6. ^**P<0.01 and ^***P<0.001.

Abbreviations: CCK-8, Cell Counting Kit-8; PI, propidium iodide; IHC, immunohistochemistry; FITC, fluorescein isothiocyanate.