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. 2018 Nov 27;10:6431–6444. doi: 10.2147/CMAR.S182980

Figure 5.

Figure 5

Effect of H. pylori on NLRP6 expression.

Notes: (A and B) BGC-823 and HGC-27 cells were infected with H. pylori at an MOI of 0, 25, or 100 for 24 hours. The protein (A) and mRNA (B) levels of NLRP6 were detected. (C) The potential binding sites in the promoter of the NLRP6 genes for FOXO3 were shown based on the online program ALGGEN – PROMO. (D) The levels of p-AKT, AKT, p-FOXO3, and FOXO3 in gastric cancer cells with H. pylori infection were determined by Western blot. (E–G) BGC-823 and HGC-27 cells were transfected with siFOXO3 and siNC for 48 hours. Western blot (E), real-time PCR (F), and luciferase reporter assays (H) were performed. (H–J) Gastric cancer cells were treated with 20 µM of LY294002 of DMSO followed by infection with H. pylori (MOI of 100) for 24 hours. Western blot (H), real-time PCR (I), and luciferase reporter assays (J) were performed. *P<0.05, **P<0.01, and ***P<0.001.

Abbreviations: H. pylori, Helicobacter pylori; TSS, transcription start site; siFOXO3, FOXO3 siRNA; siNC, negative control siRNA; DMSO, dimethyl sulfoxide; MOI, multiplicity of infection; LY, LY294002.