Figure 4.

Afatinib decreased NRG1 pathway stimulation in CS LC cells.

Notes: (A) H1299 and (B) HCC78 cells, as well as corresponding parent cells, received the supplement of 500 nM ceritinib, 200 nM afatinib, or both for 2 days. WB was used to evaluate the expression of certain proteins. (C–D) RT-PCR was used to assess NRG1 transcription. Data are presented as mean ± SD from three independent experiments. **P<0.01, ceritinib + afatinib vs ceritinib.

Abbreviations: CS, ceritinib sensitivity; LC, lung cancer; WB, Western blot.