Figure 4.
Decreased mitochondrial oxidative phosphorylation-OXPHOS complex III-linked and uncoupled complex III oxidation rate in peripheral blood mononuclear cells of prostate cancer patients undergoing radiation therapy (n=15).
Notes: The X-axis indicates time points – day 0 (prior radiation therapy) and day 21 (during radiation therapy); the Y-axis represents cell respiration rate (pmol O2/sec/106 cells); OXPHOS-complex III-linked, coupled complex III rate was measured as the ADP-stimulated respiration with DHQ, a substrate for complex III, representing respiration rate of coupled complex III. Uncoupled complex III was determined after the addition of an uncoupler, FCCP. Both coupled and uncoupled complexes III oxidation rates were significantly decreased at day 21 of XRT from day 0 (P<0.05).
Abbreviations: ADP, adenosine diphosphate; DHQ, duroquinol; FCCP, carbonylcyanide-p-trifluoromethoxyphenylhydrazone; OXPHOS, oxidative phosphorylation; XRT, radiation therapy.