Table 2.
Integrated mitochondrial function in peripheral blood mononuclear cells from prostate cancer patients undergoing radiation therapy (n=15)
| Indicators of integrated mitochondrial function | Mean ± SEM | ||
|---|---|---|---|
| pmol O2/sec/106 cells | Day 0 (prior radiotherapy) | Day 21 (during radiotherapy) | Difference between day 0 and day 21 |
| Protocol 1 | |||
| Intact cell respiration | 6.96±0.41 | 5.65±0.51 | ↓ 1.31 |
| Intact cell respiration + (m+p) | 7.59±0.52 | 6.81±0.64 | ↓ 0.78 |
| OXPHOS (CI-linked) | 7.61±0.92 | 6.93±0.66 | ↓ 0.68 |
| OXPHOS (CI + CII-linked) | 17.55±1.73 | 13.55±0.88 | ↓ 4.0* |
| Maximal oxidative capacity (uncoupler, FCCP) | 19.78±2.03 | 16.19±1.04 | ↓ 3.59 |
| Uncoupled CI | 7.62±1.91 | 6.83±1.72 | ↓ 0.79 |
| Uncoupled CII | 11.54±1.31 | 8.9±0.71 | ↓ 2.64* |
| Uncoupled CIV | 209.43±25.88 | 205.36±25.14 | ↓ 4.1 |
| Reserve capacity (Difference between OXPHOS [CI + CII-linked] and maximal oxidative capacity) | 2.23±0.16* | 2.64±0.21* | ↑ 0.41 |
| Citrate synthase (nmol/min/mg/106 cells) | 3.91±1.05 | 3.12±0.51 | ↓ 0.79 |
| Protocol 2 | |||
| Intact cell respiration | 6.21±0.48 | 6.71±0.89 | ↑ 0.50 |
| Intact cell respiration + (m+pal) | 6.34±0.59 | 7.02±1.01 | ↑ 0.68 |
| OXPHOS (fatty acid oxidation) | 5.35±0.65 | 6.75±0.91 | ↑ 1.4 |
| OXPHOS (coupled CIII; DHQ) | 28.62±2.92 | 24.63±2.61 | ↓ 3.99* |
| Uncoupled Complex III (uncoupler, FCCP) | 29.7±3.21 | 25.64±2.72 | ↓ 4.06* |
| Reserve capacity (Difference between OXPHOS [coupled CIII-linked; DHQ] and uncoupled complex III | 1.08±0.41 | 1.01±0.33 | ↓0.07 |
| Citrate synthase (nmol/min/mg/106 cells) | 4.94±1.26 | 5.75±1.17 | ↑ 0.81 |
Notes: In protocol 1, intact cell respiration + (m+p) was measured after the addition of malate and pyruvate before permeabilization; in protocol 2, intact cell respiration + (m+pal) was determined after the addition of malate and palmitoylcarnitine before permeabilization. Data are presented as mean ± standard error of mean.
P<0.05.
Abbreviations: CI, complex I; CII, complex II; CIII, complex III; CIV, complex IV; DHQ, duroquinol; FCCP, carbonylcyanide-p-trifluoromethoxyphenylhydrazone; m, malate; OXPHOS, oxidative phosphorylation; p, pyruvate; pal, palmitoylcarnitine; sec, second; SEM, standard error of mean.