Fig. 3.

Hypoxic HCT116 cells are susceptible to TH588 treatment. Hypoxic samples were placed in a hypoxia workstation for 12 h before treatment to adapt the cells to low oxygen concentrations. TH588 was applied in the hypoxic chamber. HCT116 (a) and SW480 (b) cells were treated with TH588 in hypoxia or normoxia and incubated for 72 h. The MTT reagent was added 4 h before lysis. Graphs were normalized to the untreated and un-irradiated control. Bonferroni’s test was used as post-hoc test. c Apoptosis induction was analyzed by caspase-3 assay. The cells were treated as in A. Whole cell protein extracts were produced after 72 h. Dunnett’s test was used as post-hoc test. d Colony formation assay of cells cultured at 1% oxygen. Immediately after irradiation, the cells were treated with TH588 and incubated for 10 days. Bonferroni’s post-hoc test was used to determine significant differences between groups