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. 2018 Nov 29;18:1190. doi: 10.1186/s12885-018-5095-x

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Survival of SW480 cells, but not of HCT116 cells depends on MTH1. a Knockdown efficiency was demonstrated by Western blotting. HCT116 and SW480 cells were harvested 72 h after knockdown induction. HIF-1β and β-actin served as control genes to confirm equal loading and protein transfer. b MTT assay in response to lentiviral knockdown of MTH1. Viability was measured 72 h after knockdown induction. c Apoptosis induction was assessed by measurement of caspase-3 activity 72 h after knockdown induction. d To calculate clonogenic survival, colonies were counted 10 days after knockdown induction. Bars were normalized to the control in each group. For statistical analysis Student’s t-test was used