Fig. 7.
Inhibition of exosome generation derepressed the expression of SNAP-25 protein in the hippocampi of Tg mice. a Intraperitoneal injection of GW4869 inhibited CD63 expression in the heart of Tg mice at the age of 6 M compared with age-matched Tg mice, as measured by western blotting. PLevene = 0.419, one-way ANOVA: F = 12.778, P = 0.001; Fisher’s PLSD test: PWT:Tg = 0.002, PTg::GW4869 = 0.001. n = 5 animals for each group. b-c Intraperitoneal injection of GW4869 prevented the elevation of miR-1 level in the blood (b: PLevene < 0.001, one-way ANOVA: F = 26.140, P < 0.0001; Fisher’s PLSD test: PWT:Tg < 0.0002, PTg::GW4869 < 0.0001. n = 6 animals for each group) and hippocampi (c: PLevene < 0.001, one-way ANOVA: F = 7.756, P = 0.011; Fisher’s PLSD test: PWT:Tg = 0.004, PTg::GW4869 = 0.0181. n = 4 animals for each group) of Tg mice, as measured by qRT-PCR. d Intraperitoneal injection of GW4869 derepressed SNAP-25 expression in the hippocampi of Tg mice, as measured by western blotting. PLevene = 0.419, one-way ANOVA: F = 12.778, P = 0.001; Fisher’s PLSD test: PWT:Tg = 0.002, PTg::GW4869 = 0.001. n = 6 animals for each group. *P < 0.05 versus WT; #P < 0.05 versus Tg