KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-Phospho-Threnine (anti-pThr) | Cell Signaling Technology | Cat# 9831S |
| Rat anti-HA | Roche | Cat# 11867423001, RRID:AB_10094468 |
| Rabbit anti-myc | Cell Signaling Technology | Cat# 2278S, RRID:AB_10693332 |
| Mouse anti-MBP | New England Biolabs | Cat# E8032, RRID:AB_1559730 |
| Mouse anti-beta-Tubulin | Cell Signaling Technology | Cat# 86298, RRID:AB_2715541 |
| Rabbit anti-Luciferase | Sigma-Aldrich | Cat# L0159; RRID:AB_260379 |
| Rabbit anti-Ubiquitin | Enzo Life Sciences | Cat# BML-PW0930, RRID:AB_10998070 |
| Mouse anti-GFP | Sigma-Aldrich | Cat# 11814460001, RRID:AB_390913 |
| Rabbit anti-SDS2 (poly-clonal) | This paper | N/A |
| Mouse anti-SPL11 (mono-clonal) | This paper | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Ubiquitin (human, recombinant) (untagged) | Enzo | Cat# BML-UW8795–0005 |
| Ubiquitin mutant (all K to R) (human, recombinant) (untagged) | Enzo | Cat# BML-UW0205–1000 |
| Wheat E1 | This paper | N/A |
| Arabidopsis E2 (UBC10) | This paper | N/A |
| Lambda Protein Phosphatase (λPP) | New England Biolabs | Cat# P0753 |
| Cellulase RS | Yakult Pharmaceutical | CAS: 9012-54-8 |
| Macerozyme R-10 | Yakult Pharmaceutical | CAS: 9032-75-1 |
| Chitin | Santa Cruz Biotechnology | Cat# SC-217876, CAS: 1398-61-4 |
| flagellin22 (flg22) | GenScript | Cat# RP 19986 |
| MG115 (26S Proteasome inhibitor) | AG Scientific | Cat# M-1155, CAS: 133407-86-0 |
| Cycloheximide (CHX) | Sigma-Aldrich | Cat# C7698, CAS: 66-81-9 |
| Pro-Q Diamond Dye | Invitrogen | Cat# P33301 |
| Luminol | Sigma-Aldrich | Cat# 123072, CAS: 521-31-3 |
| Luciferin | Goldbio | Cat# LUCK-1G, CAS: 15144-35-9 |
| 3,3-Diaminobenzidine (DAB) | Sigma-Aldrich | Cat# D8001, CAS: 91-95-2 |
| PEG4000 | Sigma-Aldrich | Cat# 81240, CAS: 25322-68-3 |
| Brefeldin A (BFA) | Cell Signaling Technology | Cat# 9972, CAS: 20350-15-6 |
| Wortmannin (WM) | Cell Signaling Technology | Cat# ,9951, CAS: 19545-26-7 |
| Experimental Models: Organisms/Strains | ||
| Rice: IR64 | This paper | N/A |
| Rice: Nipponbare (NPB) | This paper | N/A |
| Rice: spl11 (IR64 background) | This paper | N/A |
| Rice: sds2 (IR64 background) | This paper | N/A |
| Rice: sds2 spl11 (IR64 background) | This paper | N/A |
| Rice: TP309spl11 (spl11 in TP309 background) | This paper | N/A |
| Rice: TP309 | This paper | N/A |
| Rice: pUbi::sds2III/TP309spl11(transgenic line) | This paper | N/A |
| Rice: pUbi::SDS2-GFP/NPB | This paper | N/A |
| Rice: pUbi::SDS2K540E-GFP/NPB | This paper | N/A |
| Rice: SDS2-ACT (SDS2 activation tagging line, Kitaake background) | This paper | N/A |
| Rice: osrlck118–1 (Dongjin Background) | This paper | N/A |
| Rice: osrlck118–2 (Dongjin Background) | This paper | N/A |
| Rice: osrlck176–1 (Dongjin Background) | This paper | N/A |
| Rice: osrlck176–2 (Dongjin Background) | This paper | N/A |
| Rice: osrlck118–1 spl11 double mutant | This paper | N/A |
| Rice: osrlck118–2 spl11 double mutant | This paper | N/A |
| Rice: osrlck176–1 spl11 double mutant | This paper | N/A |
| Rice: osrlck118–2 SDS2-ACT double mutant | This paper | N/A |
| Magnaporthe oryzae: isolates RO1 −1 and PO6–6 | This paper | N/A |
| Oligonucleotides | ||
| Primers for SDS2 qRT-PCR, see Table S2 | This paper | N/A |
| Primers for SDS2 variants, see Table S2 | This paper | N/A |
| Primers for OsRLCK118 qRT-PCR, see Table S2 | This paper | N/A |
| Primers for OsRLCK118 qRT-PCR, see Table S2 | This paper | N/A |
| Primers for OsPAL1 qRT-PCR, see Table S2 | This paper | N/A |
| Primers for OsPR5 qRT-PCR, see Table S2 | This paper | N/A |
| Primers for rice blast fungal biomass assay, see Table S2 | This paper | N/A |
| Recombinant DNA | ||
| pCAMBIA-pUbi::SDS2-GFP | This paper | N/A |
| pCAMBIA-pUbi::SDS2K540E-GFP | This paper | N/A |
| pCAMBIA-pUbi::sds2lll | This paper | N/A |
| pYBA-35S::RFP | This paper | N/A |
| pYBA-35S::OsRLCK118-GFP | This paper | N/A |
| pYBA-35S::OsRLCK176-GFP | This paper | N/A |
| PSPYNE173-SDS2 (SDS2-YN) | This paper | N/A |
| pSPYNEI73-SDS2K540E (SDS2K540E-YN) | This paper | N/A |
| pSPYCE(M)-SPL11Δ3aa (SPL11Δ3aa-YC) | This paper | N/A |
| pSPYCE(M)-OsRLCK118 (OsRLCK118-YC) | This paper | N/A |
| pSPYCE(M)-OsRLCK176 (OsRLCK176-YC) | This paper | N/A |
| pSPYCE(M)-OsRLCK185 (OsRLCK185-YC) | This paper | N/A |
| pSPYCE(M)-OsRLCK107 (OsRLCK107-YC) | This paper | N/A |
| pYBA-35S::OsRLCK118-HA | This paper | N/A |
| pYBA-35S::OsRLCK176-HA | This paper | N/A |
| pYBA-35S::OsRLCK185-HA | This paper | N/A |
| pYBA-35S::OsRLCK107-HA | This paper | N/A |
| PYBA-35S:: SDS2-HA | This paper | N/A |
| pYBA-35S:: SDS2K540E-HA | This paper | N/A |
| PYBA-35S:: OsCERK1-HA | This paper | N/A |
| pDBIeu-SDS2KD | This paper | N/A |
| pDBIeu-SDS2K540E | This paper | N/A |
| pPC86-SPL11 | This paper | N/A |
| pPC86-SPL11Δ3aa | This paper | N/A |
| pPC86-SPL11V290R | This paper | N/A |
| pPC86-SPL11-NT | This paper | N/A |
| PPC86-SPL11-CT | This paper | N/A |
| pPC86-SPL11-Arm | This paper | N/A |
| pGBKT7-SDS2KD | This paper | N/A |
| pGBKT7-SDS2K540E | This paper | N/A |
| PGADT7-SPL11 | This paper | N/A |
| pGADT7-SPL11Δ3aa | This paper | N/A |
| pGADT7-SPL11V290R | This paper | N/A |
| pGADT7-OsPUB12 | This paper | N/A |
| pGADT7- OsPUB12Δ3aa | This paper | N/A |
| pMAL-c2-SDS2KD | This paper | N/A |
| pMAL-c2-SDS2K540E | This paper | N/A |
| pMAL-c2-SPL11 | This paper | N/A |
| pMAL-c2-SPL11Δ3aa | This paper | N/A |
| pMAL-c2-OsRbohB-N (N erminus, 355 aa) | This paper | N/A |
| pGEX-6p-SDS2KD | This paper | N/A |
| pGEX-6p-SDS2K540E | This paper | N/A |
| pGEX-6p-OsRLCK118 | This paper | N/A |
| pGEX-6p-OsRLCK118K116E | This paper | N/A |
| pGEX-6p-OsRLCK176 | This paper | N/A |
| pGEX-6p-OsRLCK176K108E | This paper | N/A |
| Software and Algorithms | ||
| ImageJ | NIH | Ver 1.49a; RRID:SCR_003070 |
| Image Lab software | Bio-Rad | RRID:SCR_014210 |