Fig 4. Analysis of HIV-1 epitope-specific CD4⁺ and CD8⁺ T cell responses elicited in MVA-primed NHPs by αDCIR.HIV5pep and αCD40.HIV5pep vaccines.

PBMCs were collected from individual animals at week 10 (two weeks post MVA) and at peak response times of week 26 (2 weeks post DC-targeting vaccination) for G1 MVA αDCIR and G2 MVA αCD40. PBMC were stimulated in the presence of Brefeldin A for 6 h with pools of HIV-1 peptides corresponding to the four indicated gene regions and analyzed by flow cytometry. Each dot is the background-subtracted value for individual animals of (A) CD154⁺ CD4⁺ or (B) CD8⁺ T cells secreting IFNγ, TNFα, IL-2, or combinations thereof when stimulated with Gag p17, Gag p24, Nef and Pol peptides. Negative background subtracted values were set to zero. Responses from individual animals in the indicated groups are presented. The mid-line of the box denotes the median, and the ends of the box denote the 25^th and 75^th percentiles. The whiskers are the minimum/maximum value higher/lower than 1.5* Inter-Quartile Interval. One animal had a value (0.9%) outside the plotted scale for the CD4⁺ T cell response to Pol peptides. S4 Table shows the data corresponding to this figure.