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. 2018 Nov 30;13(11):e0207255. doi: 10.1371/journal.pone.0207255

Fig 5. MMP-9 cleaves effector T-cell chemoattractant ligands in vitro, renders them functionally inactive in T-cell migration assays.

Fig 5

(A) Overnight treatment of CXCL9, CXCL10, and CXCL11 proteins with activated MMP-9 results in protein degradation in a dose-responsive manner (B) Transient (2 hours) cleavage of CXCL9, CXCL10, and CXCL11 with activated MMP-9 renders the chemokines functionally inactive in primary, activated T-cell migration assays across multiple donors. A representative result of 3 independent experiments is shown. (C) MMP-9 cleaves CXCL9 at the C-terminus at a previously described cut site. (D) Assessment of the activity of truncated CXCL9 protein shown in (C) revealed significantly reduced activity compared to wild-type CXCL9 in the activated T-cell migration assay across multiple donors.