Table 1.
Effects of compounds 7, 8, 13–15, 17, 18, 24–26 on the aggregation of washed rabbit platelets induced by arachidonic acid (AA), collagen (Col), PAF and thrombin (Thr).
| Aggregation (%) | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Inducer | Control | Conc. (μg/mL) | 7 | 8 | 13 | 14 | 15 | 17 | 18 | 24 | 25 | 26 |
| AA | 85.9 ± 0.6 | 0.1 | - | - | 83.3 ± 2.0 | - | - | - | ||||
| (100 μM) | 0.2 | 85.6 ± 0.2 | - | 33.7 ± 19.1 † | 80.6 ± 3.6 | 80.9 ± 1.7 † | - | - | ||||
| 0.5 | 17.6 ± 12.5 ‡ | 82.9 ± 1.4 | 16.3 ± 10.1 ‡ | 82.1 ± 7.3 | 79.8 ± 3.8 † | 16.6 ± 14.4 ‡ | 1.4 ± 1.2 ‡ | - | 85.6 ± 1.7 | |||
| 1.0 | 0.0 ± 0.0 ‡ | 0.0 ± 0.0 ‡ | 0.0 ± 0.0‡ | 18.1 ± 3.8 ‡ | 2.7 ± 2.3 ‡ | 0.0 ± 0.0 ‡ | 0.0 ± 0.0 ‡ | - | 44.4 ± 21.1 * | |||
| 2.0 | - | - | - | 0.0 ± 0.0 ‡ | 0.0 ± 0.0 ‡ | - | 86.1 ± 0.7 | 34.9 ± 18.3 ‡ | ||||
| 5.0 | - | - | - | - | 41.5 ± 20.8 * | 0.0 ± 0.0 ‡ | ||||||
| 10.0 | - | - | - | - | 27.9±16.4 ‡ | - | ||||||
| 20.0 | - | - | - | - | 0.0 ± 0.0 ‡ | - | ||||||
| 50.0 | - | - | - | - | - | - | 84.8 ± 0.5 | |||||
| 100.0 | 0.0 ± 0.0 ‡ | 0.0 ± 0.0 ‡ | 0.0 ± 0.0 ‡ | 0.0 ± 0.0 ‡ | 0.0 ± 0.0 ‡ | 0.0 ± 0.0 ‡ | 0.0 ± 0.0 ‡ | 0.0 ± 0.0 ‡ | 0.0 ± 0.0 ‡ | 32.1 ± 16.7 † | ||
| Col | 87.1 ± 1.0 | 0.5 | 83.3 ± 0.7 * | - | - | |||||||
| (10 μg/mL) | 1.0 | - | - | 84.4 ± 2.9 | 71.9 ± 4.7 ‡ | - | - | |||||
| 2.0 | 78.5 ± 2.7 | - | 69.6 ± 9.9 † | 84.2 ± 3.7 | 30.6 ± 10.8 ‡ | 82.5 ± 4.0 | ||||||
| 5.0 | 37.4 ± 15.6 ‡ | 86.3 ± 1.3 | 40.6 ± 17.9 ‡ | 76.8 ± 4.8 † | 77.7 ± 1.9 ‡ | 0.0 ± 0.0 ‡ | 25.4 ± 14.0 ‡ | |||||
| 10.0 | 4.6 ± 4.0 ‡ | 54.8 ± 18.0 * | 12.6 ± 10.3 ‡ | 29.0 ± 16.9 ‡ | 20.6 ± 10.6 ‡ | 0.0 ± 0.0 ‡ | 80.1 ± 5.1 | |||||
| 20.0 | 0.0 ± 0.0 ‡ | 4.6 ± 4.0 ‡ | 0.0 ± 0.0 ‡ | 0.0 ± 0.0 ‡ | 0.0 ± 0.0 ‡ | 77.6 ± 4.0 † | 54.7 ± 15.6 * | |||||
| 50.0 | - | 0.0 ± 0.0 ‡ | - | 33.0 ± 17.2 ‡ | 47.2 ± 13.7 ‡ | |||||||
| 100.0 | 0.0 ± 0.0 ‡ | 0.0 ± 0.0 ‡ | 0.0 ± 0.0 ‡ | 0.0 ± 0.0 ‡ | 0.0 ± 0.0 ‡ | 0.0 ± 0.0 ‡ | 0.0 ± 0.0 ‡ | 3.8 ± 3.3 ‡ | 15.5 ± 8.9 ‡ | 89.5 ± 1.8 | ||
| PAF | 88.6 ± 1.8 | 20.0 | 84.3 ± 3.6 | |||||||||
| (2 nM) | 50.0 | 83.9 ± 1.0 | 83.0 ± 1.0 * | 83.2 ± 1.4 | 83.4 ± 1.6 | 59.9 ± 8.5 † | 84.9 ± 4.0 | |||||
| 100.0 | 55.8 ± 4.7 ‡ | 55.3 ± 11.6 † | 24.4 ± 8.6 ‡ | 10.5 ± 8.6 ‡ | 0.0 ± 0.0 ‡ | 55.8 ± 9.2 † | 84.5 ± 2.4 | 84.9 ± 1.5 | 85.6 ± 1.3 | |||
| Thr (0.1 μg/mL) | 92.3 ± 1.0 | 100.0 | 87.4 ± 1.1 | 84.5 ± 0.6 ‡ | 85.0 ± 0.5 ‡ | 84.3 ± 2.5 * | 70.5 ± 2.7 ‡ | 80.2 ± 0.9 ‡ | 85.1 ± 4.7 | 88.5 ± 1.1 * | 88.2 ± 3.6 | |
Platelets were preincubated with compounds or DMSO (0.5%, control) at 37 °C for 3 min; the inducer was then added. Values were means ± s.e.m. (n = 3–7). * p < 0.05, † p < 0.01, ‡p < 0.001 as compared with the control.