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SDS-PAGE—In house antigen (34 μg) were loaded on a 15% polyacrylamide gel and resolved with a constant current of 20 mA for approximately 2 h. Protein bands were stained with Coomassie Blue R250. Lane 1, molecular mass markers (Phosphorylase b—97 kDa; bovine serum albumin—66 kDa; ovalbumin—45 kDa; carbonic anhydrase—29 kDa; soybean trypsin inhibitor—20.1 kDa and lactalbumin—14.4 kDa); Lane 2: in-house antigen.
