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. 2012 Jun 5;17(6):6832–6839. doi: 10.3390/molecules17066832

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© 2012 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Inhibition kinetic analysis of celastrol (Cela) towards UGT2B7-catalyzed 4-MU glucuronidation. (A) Dixon plot of Cela’s inhibition towards UGT2B7-catalyzed 4-MU glucuronidation. (B) Lineweaver-Burk plot of Cela’s inhibition towards UGT2B7-catalyzed 4-MU glucuronidation. (C) Second plot using slope (obtained from Lineweaver-Burk plot) vs. the concentration of cela.