Table 2.

The binding constants (K_b) and the number of binding sites (n), for the binary and ternary systems at pH = 7.4 and T = 298 K.

System	K b ( L mol−1)	n	R	K b (L mol−1)	n	R
	λ ex = 280 nm	λ ex = 280 nm		λ ex = 295 nm	λ ex = 295 nm
hTf-ROP	K b 1 = (1.69 ± 0.03) × 106	N1 = 0.82	0.997	(3.75 ± 0.03) × 108	1.52	0.998
	K b 2 = (1.86 ± 0.03) × 105	N2 = 0.59	0.998
hTf-ASA	(7.83 ± 0.02) × 103	0.74	0.999	(5.51 ± 0.02) × 103	0.78	0.998
(hTf-ASA)-ROP	(8.42 ± 0.02) × 105	1.14	0.998	(5.44 ± 0.02) × 106	1.20	0.996
(hTf-ROP)-ASA	(2.06 ± 0.02) × 104	0.89	0.997	(3.58 ± 0.02) × 104	1.19	0.995