Table 3.
Effect of the injection solvent on the determination of the final carotenoid content of transgenic maize. A homogeneous lyophilizedmaize sample (TM1) was used for all experiments.
| Injection solvent a | Anther μg/g DW | Adonix μg/g DW | Lut+zeax μg/g DW | α-Crypt μg/g DW |
|---|---|---|---|---|
| Acetone 100% | 0.12 | 1.93 | 2.20 | 0.98 |
| Mobile phase b–acetone 6.7:3.3, v/v | 0.24 | 4.71 | 4.49 | 1.36 |
| Mobile phase b 100% | 0.20 | 4.48 | 3.89 | 1.27 |
| Mobile phase b–acetone 7.5:2.5, v/v | 0.22 | 4.39 | 4.20 | 1.26 |
| ACN–MeOH– iPrOH 8.5:1:0.5, v/v/v | 0.15 | 3.31 | 3.05 | 1.12 |
a Chromatographic conditions are described in Section 3.3.5; b Solvent A, ACN: MeOH 7:3, v/v. Abbreviations: Anther, antheraxanthin; Adonix, adonixanthin; Lut, lutein; Zeax, zeaxanthin; α-Crypt, α-cryptoxanthin.