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. 2012 Nov 22;17(12):13769–13786. doi: 10.3390/molecules171213769

Figure 3.

Figure 3

RMO inhibited the production of LPS-induced pro-inflammatory cytokine in RAW 264.7 cells. (A) RAW 264.6 cells were pretreated with RMO for 1 h, and then stimulated with LPS (1 μg/mL). Culture media were collected after 24 h in order to measured TNF-α and IL-6 concentrations using ELISA. Data are presented as the means ± S.D. of three independent experiments. *, #Significantly different from the no treatment and LPS-treated control, respectively (p < 0.05). (B) The cells were incubated with RMO in the presence or absence of LPS for 24 h. Then the cells were harvested and whole cell extracts were prepared for Western blot analysis for the indicated proteins. The results are expressed as the means ± S.D. for three separate experiments, each with three replicates.