Table 1.
HPLC-ESI-MS and corresponding CID data of saponins in different parts of P. notoginseng.
| No | Peak identification | RT(min) | MS ( m/z) | CID( m/z) | UG | a | b | c | d | |
|---|---|---|---|---|---|---|---|---|---|---|
| [M−H]− | [M+Cl]− | |||||||||
| 1 | Yesanchinoside H | 7.2 | 1093 | 1129 | 961[M−H-Xyl]−, 931[M−H-Glc]−, 799[M−H-Xyl-Glc]−, 637[M−H-Xyl-2Glc]−, 475 [M−H-Xyl-2Glc]− | + | - | - | - | - |
| 2 | Notoginsenoside R3/R6/N/ 20-O-glucoginsenoside Rf | 8.4 | 961 | 997 | 799[M−H-Glc]−, 637[M−H-2Glc]−, 475[M−H-3Glc]− | + | - | - | - | - |
| 3 | Notoginsenoside R3/R6/N/ 20-O-glucoginsenoside Rf | 10.6 | 961 | 997 | 799[M−H-Glc]−, 637[M−H-2Glc]−, 475[M−H-3Glc]− | + | - | - | - | - |
| 4 | Notoginsenoside R3/R6/N/ 20-O-glucoginsenoside Rf | 11.2 | 961 | 997 | 799[M−H-Glc]−, 637[M−H-2Glc]−, 475[M−H-3Glc]− | + | - | - | - | - |
| 5 | Notoinsenoside R1 | 11.5 | 931 | 967 | 799[M−H-Xyl]−, 769[M−H-Glc]−, 751[M−H-Glc-H2O]−, 637[M−H-Glc-Xyl]−, 619[M−H-Glc-Xyl-H2O]−, 475[M−H-Xyl-2Glc]− | + | + | + | + | + |
| 6 | Notoinsenoside R1 isomer | 12.6 | 931 | 967 | 799[M−H-Xyl]−, 769[M−H-Glc]−, 637[M−H-Glc-Xyl]−, 475[M−H-Xyl-2Glc]− | + | - | - | - | - |
| 7 | Ginsenoside Rg1 | 13.6 | 799 | 835 | 637[M−H-Glc]−, 619[M−H-Glc-H2O]−, 475[M−H-2Glc]− | + | + | + | + | + |
| 8 | Ginsenoside Re | 13.6 | 945 | 981 | 799[M−H-Rha]−, 783[M−H-Glc]−, 765.6[M−H-Glc-H2O]−, 637[M−H-Rha-Glc]−, 619[M−H-Rha-Glc-H2O]−, 475[M−H-Rha-2Glc]− | + | + | + | + | + |
| 9 | Notoginsenoside E | 15.3 | 979 | 1015 | 817[M−H-Glc]−, 799[M−H-Glc-H2O] | + | - | - | - | - |
| 10 | Notoginsenoside A | 15.5 | 1123 | 1159 | - | + | - | - | - | - |
| 11 | Ginsenoside G | 17.9 | 959 | 996 | 797[M-Glc], 635[M-2Glc]−, 473[M-3Glc]− | + | - | - | - | - |
| 12 | Notoginsenoside R3/R6/N/ 20-O-Glucoginsenoside Rf | 21.9 | 961 | 998 | 799[M−H-Glc]−, 637[M−H-2Glc]−, 619[M−H-2Glc-H2O]−, 475[M−H-3Glc]− | + | - | - | - | - |
| 13 | Notoginsenoside R4 | 26.0 | 1239 | 1276 | 1107[M−H-pen]−, 945[M−H-xyl-Glc]−, 783[M−H-xyl-2Glc]−, 621[M−H-xyl-3Glc], 459[M−H-xyl-4Glc]− | + | - | - | - | - |
| 14 | Ginsenoside Rf | 26.1 | 799 | 835 | 637[M−H-Glc]−, 619[M−H-Glc-H2O]−, 475[M−H-2Glc]−, | + | - | + | - | - |
| 15 | Chikusetsusaponin L5 | 27.2 | 901 | 937 | 769[M−H-Xyl]−, 637[M−H-Xyl-Ara]−, 475[M−H-Xyl-Ara-Glc]− | + | - | + | + | + |
| 16 | Notoginsenoside Fa | 27.3 | 1239 | 1275 | 1107[M−H-Xyl]−, 1089[M−H-Xyl-H2O]−, 945[M−H-Xyl-Glc]−, 783[M−H-Xyl-2Glc]−, 621[M−H-Xyl-3Glc]−, 459[M−H-Xyl-4Glc]− | + | - | + | + | + |
| 17 | Notoinsenoside Q/S | 27.7 | 1341 | 1377 | 1209[M−H-Xyl]−, 1192[M−H-Xyl-H2O]−, 1077[M−H-2Pen]−, 945[M−H-3Pen]−, 783[M−H-3Pen-Glc]−, 621[M−H-3Pen-2Glc]−, 459[M−H-3Pen-3Glc]− | - | - | - | - | + |
| 18 | Notoginsenoside I | 27.7 | 1091 | 1127 | 929[M−H-Glc]−, 767[M−H-2Glc]−, 605[M−H-3Glc]−, 443[M−H-3Glc]− | + | - | - | - | - |
| 19 | Notoinsenoside R2 | 28.0 | 769 | 705 | 637[M−H-Xyl]−, 619[M−H-Xyl-H2O]−, 475[M−H-Xyl-Glc]− | + | - | - | - | - |
| 20 | Ginsenoside Fc/Ra1/Ra2 | 28.1 | 1209 | 1245 | 1077[M−H-Xyl]−, 1047[M−H-Glc]−, 1027[M−H-Glc-H2O]−, 945[M−H-Xyl-arab]−,915[M−H-Xyl-Glc]−, 897[M−H-Xyl-Glc-H2O]−, 783[M−H-Xyl-arab-Glc]− 621[M−H-Xyl-arab-2Glc]−, 459[M−H-Xyl-arab-3Glc]− | - | - | - | - | + |
| 21 | Didehydroginsenoside Rb1 | 28.2 | 1105 | 1141 | 943[M−H-Glc]−, 781[M−H-2Glc]−, 619[M−H-3Glc]−, 457[M−H-4Glc]− | + | - | - | - | - |
| 22 | Ginsenoside Fc/Ra1/Ra2 | 28.9 | 1209 | 1245 | 1077[M−H-Xyl]−, 1059[M−H-Xyl-H2O]−, 1047[M−H-Glc]−, 945[M−H-Xyl-arab]−,915[M−H-Xyl-Glc]−, 783[M−H-Xyl-arab-Glc]−, 621[M−H-Xyl-arab-2Glc]−, 459[M−H-Xyl-arab-3Glc]− | - | - | + | + | + |
| 23 | Notoinsenoside Q/S | 29.1 | 1341 | 1377 | 1209[M−H-Xyl]−, 1077[M−H-2Pen]−, 945[M−H-3Pen]−, 783[M−H-3Pen-Glc]−, 621[M−H-3Pen-2Glc]−, 459[M−H-3Pen-3Glc]− | - | - | - | - | + |
| 24 | Ginsenoside Ra3 | 29.5 | 1239 | 1275 | 1107[M−H-Xyl]−, 1089[M−H-Xyl-H2O]−, 945[M−H-Xyl-Glc]−, 783[M−H-Xyl-2Glc]−, 621[M−H-Xyl-3Glc]−, 459[M−H-Xyl-4Glc]− | + | - | - | - | - |
| 25 | Ginsenoside Rb1 | 29.6 | 1107 | 1143 | 945 [M−H-Glc]−, 783[M−H-2Glc]−, 621[M−H-3Glc]−, 459[M−H-4Glc]− | + | + | + | + | + |
| 26 | Ginsenoside Rg2 | 30.2 | 783 | 819 | 637[M−H-Rha]−, 619[M−H-Rha-H2O]−, 475[M−H-Rha-Glc]− | + | + | + | - | - |
| 27 | Ginsenoside Rh1 | 30.9 | 637 | 673 | 475[M−H-Glc]− | + | - | - | - | - |
| 28 | Ginsenoside Rc | 31.5 | 1077 | 1113 | 945[M−H-Araf]−, 915[M−H-Glc]−, 783[M−H-Araf-Glc]−, 765[M−H-Araf-Glc-H2O]−, 621[M−H-Araf-2Glc]−, 603[M−H-Araf-2Glc-H2O]−, 459[M−H-Araf-3Glc]− | + | + | + | + | + |
| 29 | Ginsenoside Fc/Ra1/Ra2 | 31.6 | 1209 | 1245 | 1077[M−H-Xyl]−, 1047[M−H-Glc]−, 945[M−H-2Xyl/(-Xyl-Ara)]−, |783[M−H-(2Xyl-Glc)/(-Xyl-Ara-Glc)]− | + | - | - | - | - |
| 30 | Ginsenoside Rb2 | 34.3 | 1077 | 1113 | 945[M−H-Xyl]−, 915[M−H-Glc]−, 783[M−H-Araf-Glc]−, 765[M−H-Xyl-Glc-H2O]−, 621[M−H-Xyl-2Glc]−, 603[M−H-Xyl-2Glc-H2O]−, 459[M−H-Xyl-3Glc]− | + | + | + | + | + |
| 31 | Ginsenoside Rb3 | 35.0 | 1077 | 1113 | 945[M−H-Xyl]−, 915[M−H-Glc]−, 783[M−H-Araf-Glc]−, 765[M−H-Xyl-Glc-H2O]−, 621[M−H-Xyl-2Glc]−, 603[M−H-Xyl-2Glc-H2O]−, 459[M−H-Xyl-3Glc]− | + | + | + | + | + |
| 32 | Notoginsenoside L | 35.5 | 1077 | 1113 | 945[M−H-Xyl]−, 915[M−H-Glc]−, 783[M−H-Xyl-Glc]−, 765[M−H-Xyl-Glc-H2O]−, 621[M−H-Xyl-2Glc]−, 603[M−H-Xyl-2Glc-H2O]−,459[M−H-Xyl-3Glc]− | + | + | + | + | + |
| 33 | Didehydroginsenoside Rd | 35.7 | 943 | 979 | 781[M−H-Glc]−, 619[M−H-2Glc]−, 457[M−H-3Glc]− | + | - | - | - | - |
| 34 | Ginsenoside F1 | 36.3 | 637 | 673 | 475[M−H-Glc]− | + | - | - | - | - |
| 35 | Ginsenoside Rd | 37.0 | 945 | 981 | 783[M−H-Glc]−, 765[M−H-Glc-H2O]−, 621[M−H-2Glc]−, 603[M−H-2Glc-H2O]−, 459[M−H-3Glc]− | + | + | + | + | + |
| 36 | Notoginsenoside K | 38.3 | 945 | 981 | 783[M−H-Glc]−, 775[M−H-Glc-H2O]−, 621[M−H-2Glc]−, 459[M−H-3Glc]− | + | + | + | + | + |
| 37 | Notoginsenoside O/P | 38.6 | 1047 | 1083 | 915[M−H-Xyl]−, 897[M−H-Xyl-H2O]−, 783[M−H-2Xyl]−, 621 [M−H-2Xyl-Glc]−,459[M−H-2Xyl-2Glc]− | - | - | - | + | + |
| 38 | Gypenoside IX/Notoginsenoside Fe/isomer | 39.5 | 915 | 951 | 783[M-pen]−, 621[M-pen-glc]−, 459[M-pen-2glc]− | + | - | - | + | + |
| 39 | Gypenoside IX/ Notoginsenoside Fe/isomer | 39.7 | 915 | 951 | 783[M-pen]−, 621[M-pen-glc]−, 459[M-pen-2glc]− | + | - | + | + | + |
| 40 | Ginsenoside F2 | 42.1 | 783 | 819 | 621[M−H-Glc]−, 603[M−H-Glc-H2O]−, 459[M−H-2Glc]− | + | - | - | - | - |
| 41 | Ginsenoside Rg3 | 44.1 | 783 | 819 | a621[M−H-Glc]−, 603[M−H-Glc-H2O]−, 459[M−H-2Glc]− | + | - | + | + | + |
UG, underground parts of P. notoginseng (including rhizome, root and fibre root); a, Seed; b, Stem; c, Leaf; d, Flower. -, Not detected; +, Detected.