(a) UPLC-PDA chromatograms (photodiode array range: 200–500 nm) showing treatment-related variations (Concentration study). Dispersive liquid-liquid microextraction (DLLME) samples of tobacco cells treated with different ergosterol concentrations (from bottom to top: 0, 50, 150, 300 and 1000 nM) and incubated for 18 h. (b) UPLC-PDA chromatograms (photodiode array range: 200–500 nm) showing time-related variations. DLLME extracts of tobacco cells treated with 300 nM ergosterol and incubated for different time period (from bottom to top: 0, 6, 12, 18 and 24 h NT) and the top last chromatogram is a non-treated sample incubated for 24 h (24 h NT).