Figure 1.

Formononetin inhibited IL-1β-induced apoptosis in INS-1cells. (A) Cells were treated with the indicated concentrations of formononetin and IL-1β for 24 h, analyzed by MTT assay. (B) Cells werestained with Annexin V-FITC and PI, analyzed by flow cytometry. Data are expressed as % of Annexin V-FITC-positiveand PI-negative cells (early stage of apoptosis). (C) Tunel staining of apoptotic cells, IL-1β (50ng/mL) and formononetin (1 μmol/L). Apoptotic cells were marked with green fluorescence, the nuclei of cells are stained by blue fluorescence. Values are the means ± SD (n = 3) of three individual experiments. * p < 0.05, ** p < 0.01 vs. ctr(DMSO); ^# p < 0.05, ^## p < 0.01 vs. group treated with IL-1β excluding formononetin.