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. 2012 Nov 12;17(11):13424–13438. doi: 10.3390/molecules171113424

Figure 4.

Figure 4

ROS production and NBT-positive cells induced by ISL. The cells were cultured with 8 μg/mL of ISL alone or in combination with NAC (500 μM), BSO (200 μM), SOD (200 μM), or tempol (200 μM) for 72 h, respectively. (A) Intracellular ROS levels were measured as described in the methods section. (B) Cell differentiation was determined and expressed as the percentage of NBT-positive cells relative to the number of viable cells (100%).(C) The levels of CD11b and CD14 mRNA expression. GAPDH was used as an internal control.