Table 1.
1H-, 13C-NMR and HMBC spectral data of compound 1 (in DMSO, δ in ppm, J in Hz).
| Structure | Position | Compound 1 | Isorhamnetin 3- O-β-D-glucoside-7-O-α-L-rhamnoside | |||
|---|---|---|---|---|---|---|
| δ H | δ C | HMBC | δ H | δ C | ||
| Flavonol group | 2 | 156.79 | 156.9 | |||
| 3 | 133.26 | 133.3 | ||||
| 4 | 177.56 | 177.6 | ||||
| 5 | 12.6 (1H, s) | 160.85 | C-5,6,10 | 161.0 | ||
| 6 | 6.43 (1H, d, 1.5 Hz) | 99.27 | C-5,7,8,10 | 6.44 (1H, d, 2.0 Hz) | 99.4 | |
| 7 | 162.73 | 161.6 | ||||
| 8 | 6.97 (1H, d, 1.5 Hz) | 94.49 | C-6,7,9,10 | 6.83(1H,d, 2.0 Hz) | 94.7 | |
| 9 | 155.97 | 156.0 | ||||
| 10 | 105.59 | 105.7 | ||||
| 1' | 120.92 | 121.0 | ||||
| 2' | 7.97 (1H, s) | 113.54 | C-2,3',6' | 7.94 (1H, s) | 113.5 | |
| 3' | 149.58 | 149.7 | ||||
| 4' | 9.87 (1H, s) | 146.90 | C-3',5' | 147.0 | ||
| 5' | 6.93 (1H, d, 8.5 Hz) | 115.20 | C-1',3' | 6.93 (1H, d, 8.5 Hz) | 115.3 | |
| 6' | 7.54 (1H, d, 8.5 Hz) | 122.19 | C-2,2',3' | 7.55 (1H, d, 8.5 Hz) | 122.4 | |
| 3'-OCH3 | 3.84 (3H, s) | 55.69 | C-3' | 3.83 (3H, s) | 55.8 | |
| Sugar at C-3 | 1'' | 5.57 (1H, d, 7.0 Hz) | 100.71 | C-3 | 5.57 (1H, d, 6.7 Hz) | 100.8 |
| 2'' | 3.0–3.8 (1H, m) | 74.32 | 3.2 (1H, m) | 74.4 | ||
| 3'' | 3.0–3.8 (1H, m) | 67.32 | 3.2 (1H, m) | 76.5 | ||
| 4'' | 3.0–3.8 (1H, m) | 69.81 | 3.1 (1H, m) | 70.1 | ||
| 5'' | 3.0–3.8 (2H, m) | 60.60 | 3.1 (1H, m) | 77.6 | ||
| 6'' | ------- | ----- | ----- | 3.57, 3.45 | 60.6 | |
| Sugar at C-7 | 1''' | 5.06 (1H, d, 6.6 Hz) | 100.11 | C-7 | 5.55 (1H, s) | 98.4 |
| 2''' | 3.0–3.8 (1H, m) | 70.00 | 3.83 (1H, s) | 70.1 | ||
| 3''' | 3.0–3.8 (1H, m) | 76.40 | 3.63 (1H, d, 3.2 Hz) | 70.3 | ||
| 4''' | 3.0–3.8 (1H, m) | 72.33 | 3.3 (1H, m) | 71.7 | ||
| 5''' | 3.0–3.8 (1H, m) | 77.45 | 3.4 (1H, m) | 69.9 | ||
| 6''' | 3.0–3.8 (2H, m) | 65.72 | 1.10 (1H, d, 6.1 Hz) | 18.0 | ||