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SDS-polyacrylamide gel electrophoresis and HPLC of F2 protein (a) F2 protein was analyzed by SDS-PAGE on 12% separating gel with a 5% stacking gel stained withCoomassie brilliant blue R-250 (Amresco). Lane 1, molecular mass marker proteins from Bio-Rad. Lane 2 and 3 were the F2 fraction and F1 fraction respectively from gel filtration chromatography on a Sephadex G-75 column; (b) HPLC chromatogram of F2 fraction.
