Figure 5.

SalA inhibits the PDGFRβ-ERK1/2 signaling cascade activated by PDGF-BB in VSMCs. Confluent VSMCs (starved for 48 h in FCS-free DMEM) were treated with SalA at different concentrations (0.003–0.1 μM) for 2 h and then were incubated in the presence of PDGF-BB for another 30 min. The cells were then lysed, and proteins were analyzed by using 12% SDS-PAGE. Western blot analysis was performed to detect the phosphorylation of PDGFR-β and ERK1/2. (A) Representative data from 3 different experiments are presented; (B,C) The phosphorylation level of these proteins is expressed as x-fold increase compared to that of normal control group. Data shown are the mean ± SEM from three independent experiments.