Table 3.
Free radical scavenging activity (EC50), total phenolic content (TPC) and total flavonoid content (TFC) of mate (Ilex paraguariensis) leaves extracts.
| Ethanol Concentration (%, v/v) | Extraction with Ethanol | Liquid CO2 with Ethanol Extraction | |
|---|---|---|---|
| 50 bar | 100 bar | ||
| EC50 (mg/mL) | |||
| 40 | 0.0157 ± 0.004 e | 0.0094 ± 0.000 b | 0.0034 ± 0.000 a |
| 50 | 0.0185 ± 0.001 f | 0.0113 ± 0.002 c | 0.0112 ± 0.001 c |
| 60 | 0.0385 ± 0.003 h | 0.0222 ± 0.001 g | 0.0132 ± 0.005 d |
| TPC (mg CAE/g d.m.) | |||
| 40 | 41.15 ± 0.18 c | 67.71 ± 0.25 f | 123.22 ± 0.85 h |
| 50 | 33.28 ± 0.66 b | 66.12 ± 0.33 f | 106.11 ± 0.73 g |
| 60 | 29.46 ± 0.23 a | 46.24 ± 0.78 d | 61.35 ± 0.55 e |
| TFC (mg CA/g d.m.) | |||
| 40 | 70.63 ± 0.13 c | 100.44 ± 0.21 f | 115.03 ± 0.28 g |
| 50 | 50.09 ± 0.21 b | 91.42 ± 0.14 e | 99.99 ± 0.33 f |
| 60 | 47.71 ± 0.09 a | 88.81 ± 0.11 d | 89.14 ± 0.25 d |
Data are expressed as the averages ± standard deviations of triplicate measurements and expressed on a dry matter basis. Different letters for EC50, TPC and TFC indicate significant difference on a level 0.05.